DNA + Protein

# cells

22,514

Mean reads/cell/amplicon

Panel uniformity

87.76%

% reads mapped to target

95.14%

Sample ?

Run ID	Example Knock Out & Protein
Analyte	DNA + Protein
DNA panel name	mirabel_dev
DNA panel size	49 amplicons
Reference genome	hg38
Pipeline version	0.3.2b15
Date analyzed	Sep-21-2023

Sequencing (DNA) ?

# total read pairs	86.18M
Read quality (Q30)	90.58 %
% read pairs trimmed	98.99 %
% read pairs with valid barcodes	96.12 %

Mapping ?

% reads mapped to genome	95.41 %
% reads mapped to target	95.14 %

Cell calling ?

# cells	22,514
Panel uniformity	87.76 %
Mean reads/cell/amplicon	67

Panel uniformity summary

Amplicon name	Median normalized counts	Mean reads	Low performer
synthego_panel_AMP1	2.15	72.71	False
synthego_panel_AMP3	0.19	11.64	True
synthego_panel_AMP5	2.73	88.61	False
synthego_panel_AMP6	1.18	38.38	False
synthego_panel_AMP7	3.13	102.53	False
synthego_panel_AMP8	1.22	40.47	False
synthego_panel_AMP9	1.10	36.25	False
synthego_panel_AMP10	0.78	26.68	False
synthego_panel_AMP11	0.00	0.42	True
synthego_panel_AMP12	2.01	65.29	False
synthego_panel_AMP14	1.34	43.79	False
synthego_panel_AMP16	4.57	150.07	False
synthego_panel_AMP17	3.84	125.55	False
synthego_panel_AMP18	1.05	34.53	False
synthego_panel_AMP19	2.31	75.95	False
synthego_panel_AMP21	2.96	96.17	False
synthego_panel_AMP22	4.06	135.41	False
synthego_panel_AMP23	3.22	104.29	False
synthego_panel_AMP26	1.66	54.96	False
synthego_panel_AMP27	0.08	4.60	True
synthego_panel_AMP28	2.06	67.36	False
synthego_panel_AMP29	2.71	89.35	False
synthego_panel_AMP30	2.46	79.96	False
synthego_panel_AMP31	0.47	17.18	False
synthego_panel_AMP33	3.26	105.23	False
synthego_panel_AMP34	3.14	102.10	False
synthego_panel_AMP35	3.92	135.11	False
synthego_panel_AMP37	2.58	85.88	False
synthego_panel_AMP41	3.45	111.25	False
synthego_panel_AMP42	0.62	22.35	False
synthego_panel_AMP43	3.72	120.76	False
synthego_panel_AMP44	0.33	12.35	True
synthego_panel_AMP45	2.72	89.14	False
synthego_panel_AMP46	2.31	74.07	False
synthego_panel_AMP47	2.50	80.98	False
synthego_panel_AMP48	3.96	128.67	False
synthego_panel_AMP50	3.76	122.92	False
synthego_panel_AMP51	0.20	12.13	True
synthego_panel_AMP53	0.76	26.77	False
synthego_panel_AMP54	0.04	2.52	True
synthego_panel_AMP55	1.06	36.90	False
synthego_panel_AMP58	1.97	63.75	False
synthego_panel_AMP60	1.37	45.48	False
synthego_panel_AMP62	0.99	32.76	False
synthego_panel_AMP65	1.87	61.08	False
synthego_panel_AMP70	2.51	81.81	False
synthego_panel_AMP72	1.57	51.05	False
synthego_panel_AMP73	1.97	63.14	False
synthego_panel_AMP74	1.05	33.93	False

Top on-target variants

Target	Variant	Modification	Total alleles	# alleles	% alleles	Total cells	# cells	% cells	% heterozygous	% homozygous
GUIDE1_ONTARGET	WT	0	32280	32214	99.8 %	16140	16074	99.59 %	0.0 %	0.0 %
GUIDE1_ONTARGET	chr2:241852753:TG/T	- 1 bp	32280	30	0.09 %	16140	30	0.19 %	0.19 %	0.0 %
GUIDE1_ONTARGET	chr2:241852785:TG/T	- 1 bp	32280	14	0.04 %	16140	14	0.09 %	0.09 %	0.0 %
GUIDE1_ONTARGET	chr2:241852760:TTG/T	- 2 bp	32280	6	0.02 %	16140	6	0.04 %	0.04 %	0.0 %
GUIDE1_ONTARGET	chr2:241852782:TC/T	- 1 bp	32280	5	0.02 %	16140	5	0.03 %	0.03 %	0.0 %
GUIDE1_ONTARGET	chr2:241852789:CG/C	- 1 bp	32280	5	0.02 %	16140	5	0.03 %	0.03 %	0.0 %
GUIDE1_ONTARGET	chr2:241852775:GC/G	- 1 bp	32280	2	0.01 %	16140	2	0.01 %	0.01 %	0.0 %
GUIDE1_ONTARGET	chr2:241852784:CT/C	- 1 bp	32280	1	0.0 %	16140	1	0.01 %	0.01 %	0.0 %
GUIDE1_ONTARGET	chr2:241852772:GA/G	- 1 bp	32280	1	0.0 %	16140	1	0.01 %	0.01 %	0.0 %
GUIDE1_ONTARGET	chr2:241852790:GGGCT/G	- 4 bp	32280	1	0.0 %	16140	1	0.01 %	0.01 %	0.0 %
GUIDE2_ONTARGET	WT	0	43742	20237	46.26 %	21871	9506	43.46 %	0.0 %	0.0 %
GUIDE2_ONTARGET	chr14:22547656:A/ACC	+ 2 bp	43742	2828	6.47 %	21871	2524	11.54 %	10.15 %	1.39 %
GUIDE2_ONTARGET	chr14:22547656:A/AC	+ 1 bp	43742	2449	5.6 %	21871	2144	9.8 %	8.41 %	1.39 %
GUIDE2_ONTARGET	chr14:22547657:C/CT	+ 1 bp	43742	871	1.99 %	21871	812	3.71 %	3.44 %	0.27 %
GUIDE2_ONTARGET	chr14:22547657:C/CG	+ 1 bp	43742	786	1.8 %	21871	732	3.35 %	3.1 %	0.25 %
GUIDE2_ONTARGET	chr14:22547656:A/ACT	+ 2 bp	43742	778	1.78 %	21871	719	3.29 %	3.02 %	0.27 %
GUIDE2_ONTARGET	chr14:22547654:AGACAT/A	- 5 bp	43742	570	1.3 %	21871	510	2.33 %	2.06 %	0.27 %
GUIDE2_ONTARGET	chr14:22547656:A/ACG	+ 2 bp	43742	408	0.93 %	21871	380	1.74 %	1.61 %	0.13 %
GUIDE2_ONTARGET	chr14:22547657:C/CGG	+ 2 bp	43742	405	0.93 %	21871	377	1.72 %	1.6 %	0.13 %
GUIDE2_ONTARGET	chr14:22547657:C/CA	+ 1 bp	43742	402	0.92 %	21871	381	1.74 %	1.65 %	0.1 %
GUIDE3_ONTARGET	WT	0	43506	43473	99.92 %	21753	21720	99.85 %	0.0 %	0.0 %
GUIDE3_ONTARGET	chr14:22550574:CA/C	- 1 bp	43506	9	0.02 %	21753	9	0.04 %	0.04 %	0.0 %
GUIDE3_ONTARGET	chr14:22550589:AT/A	- 1 bp	43506	4	0.01 %	21753	4	0.02 %	0.02 %	0.0 %
GUIDE3_ONTARGET	chr14:22550591:TG/T	- 1 bp	43506	4	0.01 %	21753	4	0.02 %	0.02 %	0.0 %
GUIDE3_ONTARGET	chr14:22550601:ATCC/A	- 3 bp	43506	3	0.01 %	21753	3	0.01 %	0.01 %	0.0 %
GUIDE3_ONTARGET	chr14:22550607:CT/C	- 1 bp	43506	2	0.0 %	21753	2	0.01 %	0.01 %	0.0 %
GUIDE3_ONTARGET	chr14:22550605:TC/T	- 1 bp	43506	2	0.0 %	21753	2	0.01 %	0.01 %	0.0 %
GUIDE3_ONTARGET	chr14:22550570:CT/C	- 1 bp	43506	2	0.0 %	21753	2	0.01 %	0.01 %	0.0 %
GUIDE3_ONTARGET	chr14:22550594:GT/G	- 1 bp	43506	2	0.0 %	21753	2	0.01 %	0.01 %	0.0 %
GUIDE3_ONTARGET	chr14:22550600:AATC/A	- 3 bp	43506	1	0.0 %	21753	1	0.0 %	0.0 %	0.0 %

Top edit combinations per group

Group	Edited targets	% cells
GUIDE2	GUIDE2_OFFTARGET7 + GUIDE2_ONTARGET	0.60 %
GUIDE2	GUIDE2_OFFTARGET4 + GUIDE2_ONTARGET	0.30 %
GUIDE2	GUIDE2_ONTARGET + GUIDE2_OFFTARGET12	0.28 %
GUIDE2	GUIDE2_ONTARGET + GUIDE2_OFFTARGET5	0.18 %
GUIDE2	GUIDE2_OFFTARGET3 + GUIDE2_ONTARGET	0.17 %
GUIDE3	GUIDE3_OFFTARGET12 + GUIDE3_OFFTARGET24	0.01 %
GUIDE3	GUIDE3_OFFTARGET20 + GUIDE3_OFFTARGET21	0.01 %
GUIDE3	GUIDE3_OFFTARGET24 + GUIDE3_OFFTARGET41	0.01 %
GUIDE3	GUIDE3_OFFTARGET1 + GUIDE3_OFFTARGET21	0.00 %
GUIDE3	GUIDE3_OFFTARGET21 + GUIDE3_OFFTARGET24	0.00 %
GUIDE1	GUIDE1_OFFTARGET3 + GUIDE1_OFFTARGET1	0.01 %

Glossary

Allele: A version of a gene that occurs in the same place on a chromosome (locus). The analysis assumes all cells are diploid, meaning two alleles are present at a given locus in a given cell. Genome editing may result in an edit in one (mono-allelic) or both (bi-allelic) alleles.

Edit: A change to the genome caused by a genome editor. Here, an edit refers to a general change, whereas a variant refers to the specific genotype of that change. In the Knockout Report, edits always refer to indels.

Genotype: the type of variant present at a given locus (location) in the genome.

Heterozygous: A particular variant present on one allele.

Homozygous: A particular variant present on two alleles.

Indel: A type of edit characterized by the insertion or deletion of nucleotides.

Monoallelic edit: An edit present on one allele.

Biallelic edit: An edit present on two alleles.

On-target: A location in the genome that is purposefully targeted for genome editing.

Off-target: A location in the genome that is an unintended target for genome editing.

Variant:The genotype of the allele. It may be wildtype (WT) or modified. A variant is described in the format of chromosome:position:reference bases/alternative bases.

Zygosity: The DNA sequence similarity across a homologous pair of chromosomes (one maternal and one paternal). In this report, their zygosity is reported at the level of edits (bi-allelic versus mono-allelic) and at the level of variants (homozygous versus heterozygous).

Default variant filters

These filters are incorporated in order to reduce false positive calls and ensure that the variants (and in turn edits) reported by the pipeline are high-quality variants with adequate genotyping and sequencing information. These filters work on individual cells. The filtering criteria are applied to the individual cells, and the genotype for the cells is removed based on the given value.

Minimum genotyping quality: Any alleles that have a genotyping quality (GQ) (calculated by GATK) below 30 are removed.

Minimum alternate allele frequency: Any alleles that have an alternate allele frequency below 10% are removed.

Minimum read depth: Any amplicon in a cell that has less than 10 read depth is removed. Read depth is inferred using the Coverage (DP) field as calculated by GATK HaplotypeCaller.

Summary Tab

Panel uniformity summary

A summary of amplicon performance. An amplicon is a targeted region of DNA that is amplified prior to sequencing (i.e., each pair of Forward & Reverse primers in a panel is for an amplicon).

Amplicon name: The name of the amplicon specified in the .amplicons file.

Mean normalized counts: The mean read percentages for that amplicon in all cells.

Mean reads: The mean reads taken by an amplicon across all cells

Low performer: A true/false indication of whether an amplicon is a low-performing amplicon or not. A low-performing amplicon is defined as an amplicon that has its mean reads below 0.2 * the mean reads per cell per amplicon.

Summary of editing

A summary of editing across all targets. The columns of this table are described below:

Group: the on-target edit and any associated predicted off-target edits (e.g., for CRISPR, a group may include both on- and off-targets edited by the same guide RNA). The Group names are designated in the "Group" column in the target group file (target_groups.csv).

Category: a designation of whether the target of interest is on-target or off-target. Each target is specified as on- or off-target in the "Category" column of the target group file (target_groups.csv).

Total alleles: The total number of alleles that meet all the criteria in the "Default variant filters" section (above) for the associated group and category. Filters are applied on each target individually and the final number shows the number of total alleles summed up across all targets in the category and group combination.

# edited alleles: number of edited alleles within the associated group and category.

% edited alleles:

The percentage of edited alleles within the associated group and category, as calculated by: (# edited alleles/ # total alleles) *100

Total cells: The total number of cells that meet all the criteria in the "Default variant filters" section (above) for the associated group and category. Filters are applied on each target individually and the final number shows the number of total cells that have at least one target passing filters in the category and group combination.

# edited cells: number of edited cells within the associated group and category.

% edited cells: the percentage of edited cells within the associated group and category, as calculated by: (# edited cells/ # total cells ) *100

Distribution of on-target alleles

The number and percentage of targeted alleles that are edited and unedited (wildtype).

Summary of editing

Group	Category	Total alleles	# edited alleles	% edited alleles	Total cells	# edited cells	% edited cells
GUIDE2	On_target	43,742	23,505	53.74 %	21,871	12,365	56.54 %
GUIDE2	Off_target	596,288	911	0.15 %	22,514	891	3.96 %
GUIDE3	On_target	43,506	33	0.08 %	21,753	33	0.15 %
GUIDE3	Off_target	840,502	1,195	0.14 %	22,514	1,157	5.14 %
GUIDE1	On_target	32,280	66	0.20 %	16,140	66	0.41 %
GUIDE1	Off_target	316,512	273	0.09 %	22,514	272	1.21 %

Top off-target variants

Target	Variant	Modification	Total alleles	# alleles	% alleles	Total cells	# cells	% cells	% heterozygous
GUIDE2_OFFTARGET9	WT	0	41326	41266	99.85 %	20663	20603	99.71 %	0.0 %
GUIDE2_OFFTARGET9	chr1:92012588:AT/A	- 1 bp	41326	18	0.04 %	20663	18	0.09 %	0.09 %
GUIDE2_OFFTARGET9	chr1:92012570:GA/G	- 1 bp	41326	17	0.04 %	20663	17	0.08 %	0.08 %
GUIDE2_OFFTARGET9	chr1:92012606:TG/T	- 1 bp	41326	15	0.04 %	20663	15	0.07 %	0.07 %
GUIDE2_OFFTARGET9	chr1:92012568:AG/A	- 1 bp	41326	5	0.01 %	20663	5	0.02 %	0.02 %
GUIDE2_OFFTARGET9	chr1:92012591:TG/T	- 1 bp	41326	4	0.01 %	20663	4	0.02 %	0.02 %
GUIDE2_OFFTARGET9	chr1:92012601:GA/G	- 1 bp	41326	1	0.0 %	20663	1	0.0 %	0.0 %
GUIDE3_OFFTARGET42	WT	0	16046	16039	99.96 %	8023	8016	99.91 %	0.0 %
GUIDE3_OFFTARGET42	chr2:10598216:CA/C	- 1 bp	16046	3	0.02 %	8023	3	0.04 %	0.04 %
GUIDE3_OFFTARGET42	chr2:10598222:CT/C	- 1 bp	16046	2	0.01 %	8023	2	0.02 %	0.02 %
GUIDE3_OFFTARGET42	chr2:10598235:GA/G	- 1 bp	16046	1	0.01 %	8023	1	0.01 %	0.01 %
GUIDE3_OFFTARGET42	chr2:10598250:G/GGGCTGGAATCCTA	+ 13 bp	16046	1	0.01 %	8023	1	0.01 %	0.01 %
GUIDE2_OFFTARGET10	WT	0	43572	43543	99.93 %	21786	21757	99.87 %	0.0 %
GUIDE2_OFFTARGET10	chr2:56779905:CA/C	- 1 bp	43572	8	0.02 %	21786	8	0.04 %	0.04 %
GUIDE2_OFFTARGET10	chr2:56779936:TG/T	- 1 bp	43572	8	0.02 %	21786	8	0.04 %	0.04 %
GUIDE2_OFFTARGET10	chr2:56779924:TG/T	- 1 bp	43572	6	0.01 %	21786	6	0.03 %	0.03 %
GUIDE2_OFFTARGET10	chr2:56779916:TA/T	- 1 bp	43572	1	0.0 %	21786	1	0.0 %	0.0 %
GUIDE2_OFFTARGET10	chr2:56779928:AT/A	- 1 bp	43572	1	0.0 %	21786	1	0.0 %	0.0 %
GUIDE2_OFFTARGET10	chr2:56779914:GT/G	- 1 bp	43572	1	0.0 %	21786	1	0.0 %	0.0 %
GUIDE2_OFFTARGET10	chr2:56779926:GA/G	- 1 bp	43572	1	0.0 %	21786	1	0.0 %	0.0 %
GUIDE2_OFFTARGET10	chr2:56779918:GAT/G	- 2 bp	43572	1	0.0 %	21786	1	0.0 %	0.0 %
GUIDE2_OFFTARGET10	chr2:56779932:GT/G	- 1 bp	43572	1	0.0 %	21786	1	0.0 %	0.0 %
GUIDE3_OFFTARGET12	WT	0	43206	43137	99.84 %	21603	21534	99.68 %	0.0 %
GUIDE3_OFFTARGET12	chr2:66773152:GT/G	- 1 bp	43206	16	0.04 %	21603	16	0.07 %	0.07 %
GUIDE3_OFFTARGET12	chr2:66773146:TG/T	- 1 bp	43206	13	0.03 %	21603	13	0.06 %	0.06 %
GUIDE3_OFFTARGET12	chr2:66773157:GA/G	- 1 bp	43206	13	0.03 %	21603	13	0.06 %	0.06 %
GUIDE3_OFFTARGET12	chr2:66773150:TG/T	- 1 bp	43206	9	0.02 %	21603	9	0.04 %	0.04 %
GUIDE3_OFFTARGET12	chr2:66773142:TC/T	- 1 bp	43206	5	0.01 %	21603	5	0.02 %	0.02 %
GUIDE3_OFFTARGET12	chr2:66773125:CT/C	- 1 bp	43206	3	0.01 %	21603	3	0.01 %	0.01 %
GUIDE3_OFFTARGET12	chr2:66773133:CT/C	- 1 bp	43206	2	0.0 %	21603	2	0.01 %	0.01 %
GUIDE3_OFFTARGET12	chr2:66773145:CT/C	- 1 bp	43206	2	0.0 %	21603	2	0.01 %	0.01 %
GUIDE3_OFFTARGET12	chr2:66773127:TC/T	- 1 bp	43206	2	0.0 %	21603	2	0.01 %	0.01 %
GUIDE2_OFFTARGET13	WT	0	43240	43203	99.91 %	21620	21583	99.83 %	0.0 %
GUIDE2_OFFTARGET13	chr2:155690975:GT/G	- 1 bp	43240	10	0.02 %	21620	10	0.05 %	0.05 %
GUIDE2_OFFTARGET13	chr2:155690958:AT/A	- 1 bp	43240	10	0.02 %	21620	10	0.05 %	0.05 %
GUIDE2_OFFTARGET13	chr2:155690947:AT/A	- 1 bp	43240	5	0.01 %	21620	5	0.02 %	0.02 %
GUIDE2_OFFTARGET13	chr2:155690949:TG/T	- 1 bp	43240	3	0.01 %	21620	3	0.01 %	0.01 %
GUIDE2_OFFTARGET13	chr2:155690951:GT/G	- 1 bp	43240	2	0.0 %	21620	2	0.01 %	0.01 %
GUIDE2_OFFTARGET13	chr2:155690953:TC/T	- 1 bp	43240	1	0.0 %	21620	1	0.0 %	0.0 %
GUIDE2_OFFTARGET13	chr2:155690946:TA/T	- 1 bp	43240	1	0.0 %	21620	1	0.0 %	0.0 %
GUIDE2_OFFTARGET13	chr2:155690943:TG/T	- 1 bp	43240	1	0.0 %	21620	1	0.0 %	0.0 %
GUIDE2_OFFTARGET13	chr2:155690941:GT/G	- 1 bp	43240	1	0.0 %	21620	1	0.0 %	0.0 %
GUIDE2_OFFTARGET1	WT	0	40922	40888	99.92 %	20461	20427	99.83 %	0.0 %
GUIDE2_OFFTARGET1	chr2:193636113:TG/T	- 1 bp	40922	19	0.05 %	20461	19	0.09 %	0.09 %
GUIDE2_OFFTARGET1	chr2:193636122:CA/C	- 1 bp	40922	3	0.01 %	20461	3	0.01 %	0.01 %
GUIDE2_OFFTARGET1	chr2:193636122:CAA/C	- 2 bp	40922	2	0.0 %	20461	2	0.01 %	0.01 %
GUIDE2_OFFTARGET1	chr2:193636132:AT/A	- 1 bp	40922	2	0.0 %	20461	2	0.01 %	0.01 %
GUIDE2_OFFTARGET1	chr2:193636125:GA/G	- 1 bp	40922	2	0.0 %	20461	2	0.01 %	0.01 %
GUIDE2_OFFTARGET1	chr2:193636120:GC/G	- 1 bp	40922	2	0.0 %	20461	2	0.01 %	0.01 %
GUIDE2_OFFTARGET1	chr2:193636110:G/GCC	+ 2 bp	40922	1	0.0 %	20461	1	0.0 %	0.0 %
GUIDE2_OFFTARGET1	chr2:193636110:G/GC	+ 1 bp	40922	1	0.0 %	20461	1	0.0 %	0.0 %
GUIDE2_OFFTARGET1	chr2:193636112:AT/A	- 1 bp	40922	1	0.0 %	20461	1	0.0 %	0.0 %
GUIDE3_OFFTARGET20	WT	0	40370	40260	99.73 %	20185	20075	99.46 %	0.0 %
GUIDE3_OFFTARGET20	chr2:238876597:ACT/A	- 2 bp	40370	64	0.16 %	20185	64	0.32 %	0.32 %
GUIDE3_OFFTARGET20	chr2:238876573:CA/C	- 1 bp	40370	21	0.05 %	20185	21	0.1 %	0.1 %
GUIDE3_OFFTARGET20	chr2:238876597:A/ACT	+ 2 bp	40370	14	0.03 %	20185	14	0.07 %	0.07 %
GUIDE3_OFFTARGET20	chr2:238876592:GA/G	- 1 bp	40370	3	0.01 %	20185	3	0.01 %	0.01 %
GUIDE3_OFFTARGET20	chr2:238876596:AAC/A	- 2 bp	40370	2	0.0 %	20185	2	0.01 %	0.01 %
GUIDE3_OFFTARGET20	chr2:238876571:AC/A	- 1 bp	40370	2	0.0 %	20185	2	0.01 %	0.01 %
GUIDE3_OFFTARGET20	chr2:238876594:GA/G	- 1 bp	40370	1	0.0 %	20185	1	0.0 %	0.0 %
GUIDE3_OFFTARGET20	chr2:238876584:AG/A	- 1 bp	40370	1	0.0 %	20185	1	0.0 %	0.0 %
GUIDE3_OFFTARGET20	chr2:238876597:A/ATT	+ 2 bp	40370	1	0.0 %	20185	1	0.0 %	0.0 %
GUIDE3_OFFTARGET37	WT	0	94	92	97.87 %	47	45	95.74 %	0.0 %
GUIDE3_OFFTARGET37	chr3:24470079:TG/T	- 1 bp	94	1	1.06 %	47	1	2.13 %	2.13 %
GUIDE3_OFFTARGET37	chr3:24470083:TC/T	- 1 bp	94	1	1.06 %	47	1	2.13 %	2.13 %
GUIDE1_OFFTARGET6	WT	0	43514	43451	99.86 %	21757	21694	99.71 %	0.0 %
GUIDE1_OFFTARGET6	chr3:29319210:TC/T	- 1 bp	43514	25	0.06 %	21757	25	0.11 %	0.11 %
GUIDE1_OFFTARGET6	chr3:29319244:CA/C	- 1 bp	43514	7	0.02 %	21757	7	0.03 %	0.03 %
GUIDE1_OFFTARGET6	chr3:29319216:AC/A	- 1 bp	43514	7	0.02 %	21757	7	0.03 %	0.03 %
GUIDE1_OFFTARGET6	chr3:29319208:AT/A	- 1 bp	43514	7	0.02 %	21757	7	0.03 %	0.03 %
GUIDE1_OFFTARGET6	chr3:29319240:AC/A	- 1 bp	43514	6	0.01 %	21757	6	0.03 %	0.03 %
GUIDE1_OFFTARGET6	chr3:29319225:AT/A	- 1 bp	43514	3	0.01 %	21757	3	0.01 %	0.01 %
GUIDE1_OFFTARGET6	chr3:29319247:AG/A	- 1 bp	43514	2	0.0 %	21757	2	0.01 %	0.01 %
GUIDE1_OFFTARGET6	chr3:29319233:TCA/T	- 2 bp	43514	2	0.0 %	21757	2	0.01 %	0.01 %
GUIDE1_OFFTARGET6	chr3:29319238:CA/C	- 1 bp	43514	2	0.0 %	21757	2	0.01 %	0.01 %
GUIDE2_OFFTARGET4	WT	0	41454	41338	99.72 %	20727	20612	99.45 %	0.0 %
GUIDE2_OFFTARGET4	chr3:124034764:TC/T	- 1 bp	41454	43	0.1 %	20727	43	0.21 %	0.21 %
GUIDE2_OFFTARGET4	chr3:124034756:TC/T	- 1 bp	41454	33	0.08 %	20727	33	0.16 %	0.16 %
GUIDE2_OFFTARGET4	chr3:124034791:TG/T	- 1 bp	41454	16	0.04 %	20727	16	0.08 %	0.08 %
GUIDE2_OFFTARGET4	chr3:124034783:GT/G	- 1 bp	41454	6	0.01 %	20727	6	0.03 %	0.03 %
GUIDE2_OFFTARGET4	chr3:124034793:GA/G	- 1 bp	41454	5	0.01 %	20727	5	0.02 %	0.02 %
GUIDE2_OFFTARGET4	chr3:124034755:CT/C	- 1 bp	41454	3	0.01 %	20727	3	0.01 %	0.01 %
GUIDE2_OFFTARGET4	chr3:124034771:AT/A	- 1 bp	41454	3	0.01 %	20727	3	0.01 %	0.01 %
GUIDE2_OFFTARGET4	chr3:124034763:CT/C	- 1 bp	41454	2	0.0 %	20727	2	0.01 %	0.01 %
GUIDE2_OFFTARGET4	chr3:124034777:GA/G	- 1 bp	41454	1	0.0 %	20727	1	0.0 %	0.0 %
GUIDE3_OFFTARGET14	WT	0	43442	43406	99.92 %	21721	21685	99.83 %	0.0 %
GUIDE3_OFFTARGET14	chr4:38203893:CA/C	- 1 bp	43442	13	0.03 %	21721	13	0.06 %	0.06 %
GUIDE3_OFFTARGET14	chr4:38203887:AT/A	- 1 bp	43442	10	0.02 %	21721	10	0.05 %	0.05 %
GUIDE3_OFFTARGET14	chr4:38203913:GA/G	- 1 bp	43442	4	0.01 %	21721	4	0.02 %	0.02 %
GUIDE3_OFFTARGET14	chr4:38203910:AG/A	- 1 bp	43442	3	0.01 %	21721	3	0.01 %	0.01 %
GUIDE3_OFFTARGET14	chr4:38203923:GA/G	- 1 bp	43442	2	0.0 %	21721	2	0.01 %	0.01 %
GUIDE3_OFFTARGET14	chr4:38203927:AC/A	- 1 bp	43442	2	0.0 %	21721	2	0.01 %	0.01 %
GUIDE3_OFFTARGET14	chr4:38203909:TA/T	- 1 bp	43442	1	0.0 %	21721	1	0.0 %	0.0 %
GUIDE3_OFFTARGET14	chr4:38203887:A/AT	+ 1 bp	43442	1	0.0 %	21721	1	0.0 %	0.0 %
GUIDE1_OFFTARGET3	WT	0	43158	43118	99.91 %	21579	21539	99.81 %	0.0 %
GUIDE1_OFFTARGET3	chr4:83901527:GA/G	- 1 bp	43158	18	0.04 %	21579	18	0.08 %	0.08 %
GUIDE1_OFFTARGET3	chr4:83901510:GA/G	- 1 bp	43158	8	0.02 %	21579	8	0.04 %	0.04 %
GUIDE1_OFFTARGET3	chr4:83901519:TG/T	- 1 bp	43158	6	0.01 %	21579	6	0.03 %	0.03 %
GUIDE1_OFFTARGET3	chr4:83901508:TG/T	- 1 bp	43158	3	0.01 %	21579	3	0.01 %	0.01 %
GUIDE1_OFFTARGET3	chr4:83901513:TG/T	- 1 bp	43158	2	0.0 %	21579	2	0.01 %	0.01 %
GUIDE1_OFFTARGET3	chr4:83901515:GA/G	- 1 bp	43158	2	0.0 %	21579	2	0.01 %	0.01 %
GUIDE1_OFFTARGET3	chr4:83901497:TG/T	- 1 bp	43158	1	0.0 %	21579	1	0.0 %	0.0 %
GUIDE3_OFFTARGET4	WT	0	41248	41175	99.82 %	20624	20551	99.65 %	0.0 %
GUIDE3_OFFTARGET4	chr4:103697203:AT/A	- 1 bp	41248	25	0.06 %	20624	25	0.12 %	0.12 %
GUIDE3_OFFTARGET4	chr4:103697186:TA/T	- 1 bp	41248	22	0.05 %	20624	22	0.11 %	0.11 %
GUIDE3_OFFTARGET4	chr4:103697190:CA/C	- 1 bp	41248	9	0.02 %	20624	9	0.04 %	0.04 %
GUIDE3_OFFTARGET4	chr4:103697208:GA/G	- 1 bp	41248	4	0.01 %	20624	4	0.02 %	0.02 %
GUIDE3_OFFTARGET4	chr4:103697212:GA/G	- 1 bp	41248	4	0.01 %	20624	4	0.02 %	0.02 %
GUIDE3_OFFTARGET4	chr4:103697192:AT/A	- 1 bp	41248	2	0.0 %	20624	2	0.01 %	0.01 %
GUIDE3_OFFTARGET4	chr4:103697185:GT/G	- 1 bp	41248	1	0.0 %	20624	1	0.0 %	0.0 %
GUIDE3_OFFTARGET4	chr4:103697203:A/AT	+ 1 bp	41248	1	0.0 %	20624	1	0.0 %	0.0 %
GUIDE3_OFFTARGET4	chr4:103697214:AG/A	- 1 bp	41248	1	0.0 %	20624	1	0.0 %	0.0 %
GUIDE3_OFFTARGET11	WT	0	43676	43652	99.95 %	21838	21814	99.89 %	0.0 %
GUIDE3_OFFTARGET11	chr4:121135145:GT/G	- 1 bp	43676	12	0.03 %	21838	12	0.05 %	0.05 %
GUIDE3_OFFTARGET11	chr4:121135121:GC/G	- 1 bp	43676	4	0.01 %	21838	4	0.02 %	0.02 %
GUIDE3_OFFTARGET11	chr4:121135124:AC/A	- 1 bp	43676	3	0.01 %	21838	3	0.01 %	0.01 %
GUIDE3_OFFTARGET11	chr4:121135132:CT/C	- 1 bp	43676	2	0.0 %	21838	2	0.01 %	0.01 %
GUIDE3_OFFTARGET11	chr4:121135142:CT/C	- 1 bp	43676	1	0.0 %	21838	1	0.0 %	0.0 %
GUIDE3_OFFTARGET11	chr4:121135129:GT/G	- 1 bp	43676	1	0.0 %	21838	1	0.0 %	0.0 %
GUIDE3_OFFTARGET11	chr4:121135130:TC/T	- 1 bp	43676	1	0.0 %	21838	1	0.0 %	0.0 %
GUIDE3_OFFTARGET1	WT	0	43668	43642	99.94 %	21834	21808	99.88 %	0.0 %
GUIDE3_OFFTARGET1	chr4:185958621:CA/C	- 1 bp	43668	11	0.03 %	21834	11	0.05 %	0.05 %
GUIDE3_OFFTARGET1	chr4:185958652:CT/C	- 1 bp	43668	2	0.0 %	21834	2	0.01 %	0.01 %
GUIDE3_OFFTARGET1	chr4:185958636:AT/A	- 1 bp	43668	2	0.0 %	21834	2	0.01 %	0.01 %
GUIDE3_OFFTARGET1	chr4:185958644:CG/C	- 1 bp	43668	2	0.0 %	21834	2	0.01 %	0.01 %
GUIDE3_OFFTARGET1	chr4:185958643:GC/G	- 1 bp	43668	2	0.0 %	21834	2	0.01 %	0.01 %
GUIDE3_OFFTARGET1	chr4:185958654:GT/G	- 1 bp	43668	2	0.0 %	21834	2	0.01 %	0.01 %
GUIDE3_OFFTARGET1	chr4:185958642:TG/T	- 1 bp	43668	1	0.0 %	21834	1	0.0 %	0.0 %
GUIDE3_OFFTARGET1	chr4:185958638:TG/T	- 1 bp	43668	1	0.0 %	21834	1	0.0 %	0.0 %
GUIDE3_OFFTARGET1	chr4:185958618:CT/C	- 1 bp	43668	1	0.0 %	21834	1	0.0 %	0.0 %
GUIDE1_OFFTARGET5	WT	0	43382	43359	99.95 %	21691	21668	99.89 %	0.0 %
GUIDE1_OFFTARGET5	chr5:16125121:TG/T	- 1 bp	43382	9	0.02 %	21691	9	0.04 %	0.04 %
GUIDE1_OFFTARGET5	chr5:16125116:CA/C	- 1 bp	43382	8	0.02 %	21691	8	0.04 %	0.04 %
GUIDE1_OFFTARGET5	chr5:16125128:TA/T	- 1 bp	43382	3	0.01 %	21691	3	0.01 %	0.01 %
GUIDE1_OFFTARGET5	chr5:16125099:TG/T	- 1 bp	43382	3	0.01 %	21691	3	0.01 %	0.01 %
GUIDE3_OFFTARGET2	WT	0	43390	43366	99.94 %	21695	21671	99.89 %	0.0 %
GUIDE3_OFFTARGET2	chr5:78266036:AC/A	- 1 bp	43390	8	0.02 %	21695	8	0.04 %	0.04 %
GUIDE3_OFFTARGET2	chr5:78266051:AT/A	- 1 bp	43390	6	0.01 %	21695	6	0.03 %	0.03 %
GUIDE3_OFFTARGET2	chr5:78266038:CA/C	- 1 bp	43390	4	0.01 %	21695	4	0.02 %	0.02 %
GUIDE3_OFFTARGET2	chr5:78266056:GA/G	- 1 bp	43390	3	0.01 %	21695	3	0.01 %	0.01 %
GUIDE3_OFFTARGET2	chr5:78266040:AT/A	- 1 bp	43390	2	0.0 %	21695	2	0.01 %	0.01 %
GUIDE3_OFFTARGET2	chr5:78266050:GA/G	- 1 bp	43390	1	0.0 %	21695	1	0.0 %	0.0 %
GUIDE3_OFFTARGET21	WT	0	42900	42847	99.88 %	21450	21398	99.76 %	0.0 %
GUIDE3_OFFTARGET21	chr6:91773341:GT/G	- 1 bp	42900	19	0.04 %	21450	19	0.09 %	0.09 %
GUIDE3_OFFTARGET21	chr6:91773346:GA/G	- 1 bp	42900	13	0.03 %	21450	13	0.06 %	0.06 %
GUIDE3_OFFTARGET21	chr6:91773322:AT/A	- 1 bp	42900	8	0.02 %	21450	8	0.04 %	0.04 %
GUIDE3_OFFTARGET21	chr6:91773316:CA/C	- 1 bp	42900	6	0.01 %	21450	6	0.03 %	0.03 %
GUIDE3_OFFTARGET21	chr6:91773330:AT/A	- 1 bp	42900	4	0.01 %	21450	4	0.02 %	0.02 %
GUIDE3_OFFTARGET21	chr6:91773353:TA/T	- 1 bp	42900	3	0.01 %	21450	3	0.01 %	0.01 %
GUIDE3_OFFTARGET26	WT	0	6056	6056	100.0 %	3028	3028	100.0 %	0.0 %
GUIDE3_OFFTARGET3	WT	0	43576	43557	99.96 %	21788	21771	99.92 %	0.0 %
GUIDE3_OFFTARGET3	chr7:3428145:AC/A	- 1 bp	43576	6	0.01 %	21788	6	0.03 %	0.03 %
GUIDE3_OFFTARGET3	chr7:3428153:CT/C	- 1 bp	43576	4	0.01 %	21788	4	0.02 %	0.02 %
GUIDE3_OFFTARGET3	chr7:3428158:AGG/A	- 2 bp	43576	4	0.01 %	21788	4	0.02 %	0.02 %
GUIDE3_OFFTARGET3	chr7:3428166:AAAG/A	- 3 bp	43576	4	0.01 %	21788	4	0.02 %	0.02 %
GUIDE3_OFFTARGET3	chr7:3428172:G/GCC	+ 2 bp	43576	4	0.01 %	21788	4	0.02 %	0.02 %
GUIDE3_OFFTARGET3	chr7:3428165:GA/G	- 1 bp	43576	2	0.0 %	21788	2	0.01 %	0.01 %
GUIDE3_OFFTARGET3	chr7:3428157:CA/C	- 1 bp	43576	2	0.0 %	21788	2	0.01 %	0.01 %
GUIDE3_OFFTARGET3	chr7:3428163:T/TCTC	+ 3 bp	43576	1	0.0 %	21788	1	0.0 %	0.0 %
GUIDE3_OFFTARGET3	chr7:3428149:AT/A	- 1 bp	43576	1	0.0 %	21788	1	0.0 %	0.0 %
GUIDE1_OFFTARGET7	WT	0	43480	43450	99.93 %	21740	21710	99.86 %	0.0 %
GUIDE1_OFFTARGET7	chr7:50937153:TC/T	- 1 bp	43480	11	0.03 %	21740	11	0.05 %	0.05 %
GUIDE1_OFFTARGET7	chr7:50937144:AT/A	- 1 bp	43480	7	0.02 %	21740	7	0.03 %	0.03 %
GUIDE1_OFFTARGET7	chr7:50937158:CT/C	- 1 bp	43480	2	0.0 %	21740	2	0.01 %	0.01 %
GUIDE1_OFFTARGET7	chr7:50937146:TA/T	- 1 bp	43480	2	0.0 %	21740	2	0.01 %	0.01 %
GUIDE1_OFFTARGET7	chr7:50937171:C/CAAGT	+ 4 bp	43480	1	0.0 %	21740	1	0.0 %	0.0 %
GUIDE1_OFFTARGET7	chr7:50937178:TTCTCCTG/T	- 7 bp	43480	1	0.0 %	21740	1	0.0 %	0.0 %
GUIDE1_OFFTARGET7	chr7:50937145:TTA/T	- 2 bp	43480	1	0.0 %	21740	1	0.0 %	0.0 %
GUIDE1_OFFTARGET7	chr7:50937152:CT/C	- 1 bp	43480	1	0.0 %	21740	1	0.0 %	0.0 %
GUIDE1_OFFTARGET7	chr7:50937152:C/CT	+ 1 bp	43480	1	0.0 %	21740	1	0.0 %	0.0 %
GUIDE1_OFFTARGET4	WT	0	43570	43536	99.92 %	21785	21751	99.84 %	0.0 %
GUIDE1_OFFTARGET4	chr7:108645223:GC/G	- 1 bp	43570	9	0.02 %	21785	9	0.04 %	0.04 %
GUIDE1_OFFTARGET4	chr7:108645215:GC/G	- 1 bp	43570	8	0.02 %	21785	8	0.04 %	0.04 %
GUIDE1_OFFTARGET4	chr7:108645219:TG/T	- 1 bp	43570	7	0.02 %	21785	7	0.03 %	0.03 %
GUIDE1_OFFTARGET4	chr7:108645226:CT/C	- 1 bp	43570	4	0.01 %	21785	4	0.02 %	0.02 %
GUIDE1_OFFTARGET4	chr7:108645236:TCA/T	- 2 bp	43570	2	0.0 %	21785	2	0.01 %	0.01 %
GUIDE1_OFFTARGET4	chr7:108645233:AT/A	- 1 bp	43570	2	0.0 %	21785	2	0.01 %	0.01 %
GUIDE1_OFFTARGET4	chr7:108645243:AC/A	- 1 bp	43570	1	0.0 %	21785	1	0.0 %	0.0 %
GUIDE1_OFFTARGET4	chr7:108645221:GA/G	- 1 bp	43570	1	0.0 %	21785	1	0.0 %	0.0 %
GUIDE3_OFFTARGET5	WT	0	29834	29814	99.93 %	14917	14897	99.87 %	0.0 %
GUIDE3_OFFTARGET5	chr7:121505276:TA/T	- 1 bp	29834	10	0.03 %	14917	10	0.07 %	0.07 %
GUIDE3_OFFTARGET5	chr7:121505280:AG/A	- 1 bp	29834	5	0.02 %	14917	5	0.03 %	0.03 %
GUIDE3_OFFTARGET5	chr7:121505294:TG/T	- 1 bp	29834	1	0.0 %	14917	1	0.01 %	0.01 %
GUIDE3_OFFTARGET5	chr7:121505281:GC/G	- 1 bp	29834	1	0.0 %	14917	1	0.01 %	0.01 %
GUIDE3_OFFTARGET5	chr7:121505273:CTT/C	- 2 bp	29834	1	0.0 %	14917	1	0.01 %	0.01 %
GUIDE3_OFFTARGET5	chr7:121505283:CT/C	- 1 bp	29834	1	0.0 %	14917	1	0.01 %	0.01 %
GUIDE3_OFFTARGET5	chr7:121505272:TC/T	- 1 bp	29834	1	0.0 %	14917	1	0.01 %	0.01 %
GUIDE2_OFFTARGET14	WT	0	43166	43135	99.93 %	21583	21552	99.86 %	0.0 %
GUIDE2_OFFTARGET14	chr8:138015596:CA/C	- 1 bp	43166	9	0.02 %	21583	9	0.04 %	0.04 %
GUIDE2_OFFTARGET14	chr8:138015610:AC/A	- 1 bp	43166	6	0.01 %	21583	6	0.03 %	0.03 %
GUIDE2_OFFTARGET14	chr8:138015613:AT/A	- 1 bp	43166	6	0.01 %	21583	6	0.03 %	0.03 %
GUIDE2_OFFTARGET14	chr8:138015625:TA/T	- 1 bp	43166	4	0.01 %	21583	4	0.02 %	0.02 %
GUIDE2_OFFTARGET14	chr8:138015609:GA/G	- 1 bp	43166	2	0.0 %	21583	2	0.01 %	0.01 %
GUIDE2_OFFTARGET14	chr8:138015622:TA/T	- 1 bp	43166	1	0.0 %	21583	1	0.0 %	0.0 %
GUIDE2_OFFTARGET14	chr8:138015621:AT/A	- 1 bp	43166	1	0.0 %	21583	1	0.0 %	0.0 %
GUIDE2_OFFTARGET14	chr8:138015612:CA/C	- 1 bp	43166	1	0.0 %	21583	1	0.0 %	0.0 %
GUIDE2_OFFTARGET14	chr8:138015617:GA/G	- 1 bp	43166	1	0.0 %	21583	1	0.0 %	0.0 %
GUIDE2_OFFTARGET7	WT	0	43504	43261	99.44 %	21752	21512	98.9 %	0.0 %
GUIDE2_OFFTARGET7	chr9:5481872:AT/A	- 1 bp	43504	154	0.35 %	21752	153	0.7 %	0.7 %
GUIDE2_OFFTARGET7	chr9:5481894:CT/C	- 1 bp	43504	39	0.09 %	21752	39	0.18 %	0.18 %
GUIDE2_OFFTARGET7	chr9:5481872:A/AT	+ 1 bp	43504	18	0.04 %	21752	18	0.08 %	0.08 %
GUIDE2_OFFTARGET7	chr9:5481879:TC/T	- 1 bp	43504	6	0.01 %	21752	6	0.03 %	0.03 %
GUIDE2_OFFTARGET7	chr9:5481879:T/TC	+ 1 bp	43504	6	0.01 %	21752	6	0.03 %	0.03 %
GUIDE2_OFFTARGET7	chr9:5481904:TC/T	- 1 bp	43504	4	0.01 %	21752	4	0.02 %	0.02 %
GUIDE2_OFFTARGET7	chr9:5481870:CA/C	- 1 bp	43504	3	0.01 %	21752	3	0.01 %	0.01 %
GUIDE2_OFFTARGET7	chr9:5481906:CTG/C	- 2 bp	43504	2	0.0 %	21752	2	0.01 %	0.01 %
GUIDE2_OFFTARGET7	chr9:5481885:TG/T	- 1 bp	43504	2	0.0 %	21752	2	0.01 %	0.01 %
GUIDE2_OFFTARGET3	WT	0	43496	43433	99.86 %	21748	21685	99.71 %	0.0 %
GUIDE2_OFFTARGET3	chr9:24807629:CA/C	- 1 bp	43496	32	0.07 %	21748	32	0.15 %	0.15 %
GUIDE2_OFFTARGET3	chr9:24807664:TC/T	- 1 bp	43496	15	0.03 %	21748	15	0.07 %	0.07 %
GUIDE2_OFFTARGET3	chr9:24807661:TA/T	- 1 bp	43496	7	0.02 %	21748	7	0.03 %	0.03 %
GUIDE2_OFFTARGET3	chr9:24807656:GC/G	- 1 bp	43496	2	0.0 %	21748	2	0.01 %	0.01 %
GUIDE2_OFFTARGET3	chr9:24807648:TG/T	- 1 bp	43496	2	0.0 %	21748	2	0.01 %	0.01 %
GUIDE2_OFFTARGET3	chr9:24807629:C/CA	+ 1 bp	43496	2	0.0 %	21748	2	0.01 %	0.01 %
GUIDE2_OFFTARGET3	chr9:24807663:AT/A	- 1 bp	43496	1	0.0 %	21748	1	0.0 %	0.0 %
GUIDE2_OFFTARGET3	chr9:24807647:GT/G	- 1 bp	43496	1	0.0 %	21748	1	0.0 %	0.0 %
GUIDE2_OFFTARGET3	chr9:24807645:ATG/A	- 2 bp	43496	1	0.0 %	21748	1	0.0 %	0.0 %
GUIDE2_OFFTARGET11	WT	0	42384	42349	99.92 %	21192	21157	99.83 %	0.0 %
GUIDE2_OFFTARGET11	chr10:1949679:TG/T	- 1 bp	42384	16	0.04 %	21192	16	0.08 %	0.08 %
GUIDE2_OFFTARGET11	chr10:1949664:TG/T	- 1 bp	42384	12	0.03 %	21192	12	0.06 %	0.06 %
GUIDE2_OFFTARGET11	chr10:1949674:AT/A	- 1 bp	42384	2	0.0 %	21192	2	0.01 %	0.01 %
GUIDE2_OFFTARGET11	chr10:1949671:CT/C	- 1 bp	42384	1	0.0 %	21192	1	0.0 %	0.0 %
GUIDE2_OFFTARGET11	chr10:1949675:T/TTGTA	+ 4 bp	42384	1	0.0 %	21192	1	0.0 %	0.0 %
GUIDE2_OFFTARGET11	chr10:1949686:GGT/G	- 2 bp	42384	1	0.0 %	21192	1	0.0 %	0.0 %
GUIDE2_OFFTARGET11	chr10:1949695:T/TCCGTCTGGTTGCTG	+ 14 bp	42384	1	0.0 %	21192	1	0.0 %	0.0 %
GUIDE2_OFFTARGET11	chr10:1949690:TG/T	- 1 bp	42384	1	0.0 %	21192	1	0.0 %	0.0 %
GUIDE2_OFFTARGET2	WT	0	43612	43582	99.93 %	21806	21776	99.86 %	0.0 %
GUIDE2_OFFTARGET2	chr11:15325811:AG/A	- 1 bp	43612	13	0.03 %	21806	13	0.06 %	0.06 %
GUIDE2_OFFTARGET2	chr11:15325792:CA/C	- 1 bp	43612	10	0.02 %	21806	10	0.05 %	0.05 %
GUIDE2_OFFTARGET2	chr11:15325800:TC/T	- 1 bp	43612	3	0.01 %	21806	3	0.01 %	0.01 %
GUIDE2_OFFTARGET2	chr11:15325823:CT/C	- 1 bp	43612	3	0.01 %	21806	3	0.01 %	0.01 %
GUIDE2_OFFTARGET2	chr11:15325815:CA/C	- 1 bp	43612	1	0.0 %	21806	1	0.0 %	0.0 %
GUIDE1_OFFTARGET8	WT	0	33242	33204	99.89 %	16621	16583	99.77 %	0.0 %
GUIDE1_OFFTARGET8	chr11:22864336:TC/T	- 1 bp	33242	9	0.03 %	16621	9	0.05 %	0.05 %
GUIDE1_OFFTARGET8	chr11:22864347:CT/C	- 1 bp	33242	6	0.02 %	16621	6	0.04 %	0.04 %
GUIDE1_OFFTARGET8	chr11:22864341:CTCT/C	- 3 bp	33242	5	0.02 %	16621	5	0.03 %	0.03 %
GUIDE1_OFFTARGET8	chr11:22864361:T/TATGTTTATCAAGGTAGGA	+ 18 bp	33242	3	0.01 %	16621	3	0.02 %	0.02 %
GUIDE1_OFFTARGET8	chr11:22864339:TC/T	- 1 bp	33242	2	0.01 %	16621	2	0.01 %	0.01 %
GUIDE1_OFFTARGET8	chr11:22864355:TC/T	- 1 bp	33242	2	0.01 %	16621	2	0.01 %	0.01 %
GUIDE1_OFFTARGET8	chr11:22864343:CTT/C	- 2 bp	33242	2	0.01 %	16621	2	0.01 %	0.01 %
GUIDE1_OFFTARGET8	chr11:22864343:CT/C	- 1 bp	33242	2	0.01 %	16621	2	0.01 %	0.01 %
GUIDE1_OFFTARGET8	chr11:22864373:TC/T	- 1 bp	33242	2	0.01 %	16621	2	0.01 %	0.01 %
GUIDE3_OFFTARGET24	WT	0	43644	43427	99.5 %	21822	21606	99.01 %	0.0 %
GUIDE3_OFFTARGET24	chr11:38714986:AT/A	- 1 bp	43644	152	0.35 %	21822	152	0.7 %	0.7 %
GUIDE3_OFFTARGET24	chr11:38714969:TG/T	- 1 bp	43644	23	0.05 %	21822	23	0.11 %	0.11 %
GUIDE3_OFFTARGET24	chr11:38714986:A/AT	+ 1 bp	43644	15	0.03 %	21822	15	0.07 %	0.07 %
GUIDE3_OFFTARGET24	chr11:38714953:CA/C	- 1 bp	43644	13	0.03 %	21822	13	0.06 %	0.06 %
GUIDE3_OFFTARGET24	chr11:38714980:TC/T	- 1 bp	43644	4	0.01 %	21822	4	0.02 %	0.02 %
GUIDE3_OFFTARGET24	chr11:38714947:CT/C	- 1 bp	43644	2	0.0 %	21822	2	0.01 %	0.01 %
GUIDE3_OFFTARGET24	chr11:38714978:CT/C	- 1 bp	43644	2	0.0 %	21822	2	0.01 %	0.01 %
GUIDE3_OFFTARGET24	chr11:38714950:CT/C	- 1 bp	43644	2	0.0 %	21822	2	0.01 %	0.01 %
GUIDE3_OFFTARGET24	chr11:38714975:CTT/C	- 2 bp	43644	1	0.0 %	21822	1	0.0 %	0.0 %
GUIDE1_OFFTARGET1	WT	0	22862	22835	99.88 %	11431	11404	99.76 %	0.0 %
GUIDE1_OFFTARGET1	chr11:71271740:GC/G	- 1 bp	22862	12	0.05 %	11431	12	0.1 %	0.1 %
GUIDE1_OFFTARGET1	chr11:71271737:GA/G	- 1 bp	22862	3	0.01 %	11431	3	0.03 %	0.03 %
GUIDE1_OFFTARGET1	chr11:71271717:TC/T	- 1 bp	22862	3	0.01 %	11431	3	0.03 %	0.03 %
GUIDE1_OFFTARGET1	chr11:71271755:CA/C	- 1 bp	22862	2	0.01 %	11431	2	0.02 %	0.02 %
GUIDE1_OFFTARGET1	chr11:71271722:TTG/T	- 2 bp	22862	2	0.01 %	11431	2	0.02 %	0.02 %
GUIDE1_OFFTARGET1	chr11:71271743:AT/A	- 1 bp	22862	1	0.0 %	11431	1	0.01 %	0.01 %
GUIDE1_OFFTARGET1	chr11:71271742:CA/C	- 1 bp	22862	1	0.0 %	11431	1	0.01 %	0.01 %
GUIDE1_OFFTARGET1	chr11:71271734:GAA/G	- 2 bp	22862	1	0.0 %	11431	1	0.01 %	0.01 %
GUIDE1_OFFTARGET1	chr11:71271721:GT/G	- 1 bp	22862	1	0.0 %	11431	1	0.01 %	0.01 %
GUIDE3_OFFTARGET32	WT	0	43576	43526	99.89 %	21788	21738	99.77 %	0.0 %
GUIDE3_OFFTARGET32	chr11:92437651:TG/T	- 1 bp	43576	16	0.04 %	21788	16	0.07 %	0.07 %
GUIDE3_OFFTARGET32	chr11:92437654:GT/G	- 1 bp	43576	9	0.02 %	21788	9	0.04 %	0.04 %
GUIDE3_OFFTARGET32	chr11:92437669:TA/T	- 1 bp	43576	9	0.02 %	21788	9	0.04 %	0.04 %
GUIDE3_OFFTARGET32	chr11:92437664:TC/T	- 1 bp	43576	6	0.01 %	21788	6	0.03 %	0.03 %
GUIDE3_OFFTARGET32	chr11:92437630:AT/A	- 1 bp	43576	6	0.01 %	21788	6	0.03 %	0.03 %
GUIDE3_OFFTARGET32	chr11:92437662:AT/A	- 1 bp	43576	2	0.0 %	21788	2	0.01 %	0.01 %
GUIDE3_OFFTARGET32	chr11:92437639:AG/A	- 1 bp	43576	1	0.0 %	21788	1	0.0 %	0.0 %
GUIDE3_OFFTARGET32	chr11:92437659:GA/G	- 1 bp	43576	1	0.0 %	21788	1	0.0 %	0.0 %
GUIDE1_OFFTARGET2	WT	0	43304	43286	99.96 %	21652	21634	99.92 %	0.0 %
GUIDE1_OFFTARGET2	chr12:43034812:TC/T	- 1 bp	43304	4	0.01 %	21652	4	0.02 %	0.02 %
GUIDE1_OFFTARGET2	chr12:43034803:CT/C	- 1 bp	43304	2	0.0 %	21652	2	0.01 %	0.01 %
GUIDE1_OFFTARGET2	chr12:43034822:A/ATCATCGAAGTCACT	+ 14 bp	43304	2	0.0 %	21652	2	0.01 %	0.01 %
GUIDE1_OFFTARGET2	chr12:43034801:TC/T	- 1 bp	43304	2	0.0 %	21652	2	0.01 %	0.01 %
GUIDE1_OFFTARGET2	chr12:43034806:TC/T	- 1 bp	43304	1	0.0 %	21652	1	0.0 %	0.0 %
GUIDE1_OFFTARGET2	chr12:43034808:CT/C	- 1 bp	43304	1	0.0 %	21652	1	0.0 %	0.0 %
GUIDE1_OFFTARGET2	chr12:43034810:CTT/C	- 2 bp	43304	1	0.0 %	21652	1	0.0 %	0.0 %
GUIDE1_OFFTARGET2	chr12:43034800:CT/C	- 1 bp	43304	1	0.0 %	21652	1	0.0 %	0.0 %
GUIDE1_OFFTARGET2	chr12:43034799:TC/T	- 1 bp	43304	1	0.0 %	21652	1	0.0 %	0.0 %
GUIDE3_OFFTARGET6	WT	0	43064	43039	99.94 %	21532	21507	99.88 %	0.0 %
GUIDE3_OFFTARGET6	chr13:79295616:TA/T	- 1 bp	43064	13	0.03 %	21532	13	0.06 %	0.06 %
GUIDE3_OFFTARGET6	chr13:79295634:CT/C	- 1 bp	43064	5	0.01 %	21532	5	0.02 %	0.02 %
GUIDE3_OFFTARGET6	chr13:79295638:GA/G	- 1 bp	43064	3	0.01 %	21532	3	0.01 %	0.01 %
GUIDE3_OFFTARGET6	chr13:79295620:CA/C	- 1 bp	43064	2	0.0 %	21532	2	0.01 %	0.01 %
GUIDE3_OFFTARGET6	chr13:79295610:GC/G	- 1 bp	43064	1	0.0 %	21532	1	0.0 %	0.0 %
GUIDE3_OFFTARGET6	chr13:79295637:TG/T	- 1 bp	43064	1	0.0 %	21532	1	0.0 %	0.0 %
GUIDE3_OFFTARGET8	WT	0	19618	19589	99.85 %	9809	9780	99.7 %	0.0 %
GUIDE3_OFFTARGET8	chr15:29970485:AC/A	- 1 bp	19618	7	0.04 %	9809	7	0.07 %	0.07 %
GUIDE3_OFFTARGET8	chr15:29970480:CA/C	- 1 bp	19618	6	0.03 %	9809	6	0.06 %	0.06 %
GUIDE3_OFFTARGET8	chr15:29970500:AT/A	- 1 bp	19618	4	0.02 %	9809	4	0.04 %	0.04 %
GUIDE3_OFFTARGET8	chr15:29970505:GA/G	- 1 bp	19618	3	0.02 %	9809	3	0.03 %	0.03 %
GUIDE3_OFFTARGET8	chr15:29970482:AT/A	- 1 bp	19618	3	0.02 %	9809	3	0.03 %	0.03 %
GUIDE3_OFFTARGET8	chr15:29970472:TA/T	- 1 bp	19618	2	0.01 %	9809	2	0.02 %	0.02 %
GUIDE3_OFFTARGET8	chr15:29970490:TC/T	- 1 bp	19618	1	0.01 %	9809	1	0.01 %	0.01 %
GUIDE3_OFFTARGET8	chr15:29970504:TG/T	- 1 bp	19618	1	0.01 %	9809	1	0.01 %	0.01 %
GUIDE3_OFFTARGET8	chr15:29970484:GA/G	- 1 bp	19618	1	0.01 %	9809	1	0.01 %	0.01 %
GUIDE3_OFFTARGET25	WT	0	40274	40209	99.84 %	20137	20072	99.68 %	0.0 %
GUIDE3_OFFTARGET25	chr15:61804119:TG/T	- 1 bp	40274	39	0.1 %	20137	39	0.19 %	0.19 %
GUIDE3_OFFTARGET25	chr15:61804103:CA/C	- 1 bp	40274	9	0.02 %	20137	9	0.04 %	0.04 %
GUIDE3_OFFTARGET25	chr15:61804107:AC/A	- 1 bp	40274	4	0.01 %	20137	4	0.02 %	0.02 %
GUIDE3_OFFTARGET25	chr15:61804099:AT/A	- 1 bp	40274	2	0.0 %	20137	2	0.01 %	0.01 %
GUIDE3_OFFTARGET25	chr15:61804122:GA/G	- 1 bp	40274	2	0.0 %	20137	2	0.01 %	0.01 %
GUIDE3_OFFTARGET25	chr15:61804131:CA/C	- 1 bp	40274	2	0.0 %	20137	2	0.01 %	0.01 %
GUIDE3_OFFTARGET25	chr15:61804123:AG/A	- 1 bp	40274	1	0.0 %	20137	1	0.0 %	0.0 %
GUIDE3_OFFTARGET25	chr15:61804126:TC/T	- 1 bp	40274	1	0.0 %	20137	1	0.0 %	0.0 %
GUIDE3_OFFTARGET25	chr15:61804122:G/GA	+ 1 bp	40274	1	0.0 %	20137	1	0.0 %	0.0 %
GUIDE3_OFFTARGET18	WT	0	1940	1936	99.79 %	970	966	99.59 %	0.0 %
GUIDE3_OFFTARGET18	chr15:70314438:ATTG/A	- 3 bp	1940	2	0.1 %	970	2	0.21 %	0.21 %
GUIDE3_OFFTARGET18	chr15:70314430:GT/G	- 1 bp	1940	1	0.05 %	970	1	0.1 %	0.1 %
GUIDE3_OFFTARGET18	chr15:70314437:AATT/A	- 3 bp	1940	1	0.05 %	970	1	0.1 %	0.1 %
GUIDE3_OFFTARGET9	WT	0	40976	40877	99.76 %	20488	20389	99.52 %	0.0 %
GUIDE3_OFFTARGET9	chr15:97989324:TA/T	- 1 bp	40976	31	0.08 %	20488	31	0.15 %	0.15 %
GUIDE3_OFFTARGET9	chr15:97989312:TG/T	- 1 bp	40976	19	0.05 %	20488	19	0.09 %	0.09 %
GUIDE3_OFFTARGET9	chr15:97989296:CA/C	- 1 bp	40976	11	0.03 %	20488	11	0.05 %	0.05 %
GUIDE3_OFFTARGET9	chr15:97989316:AT/A	- 1 bp	40976	9	0.02 %	20488	9	0.04 %	0.04 %
GUIDE3_OFFTARGET9	chr15:97989328:AC/A	- 1 bp	40976	7	0.02 %	20488	7	0.03 %	0.03 %
GUIDE3_OFFTARGET9	chr15:97989306:TA/T	- 1 bp	40976	4	0.01 %	20488	4	0.02 %	0.02 %
GUIDE3_OFFTARGET9	chr15:97989291:GT/G	- 1 bp	40976	4	0.01 %	20488	4	0.02 %	0.02 %
GUIDE3_OFFTARGET9	chr15:97989310:GA/G	- 1 bp	40976	3	0.01 %	20488	3	0.01 %	0.01 %
GUIDE3_OFFTARGET9	chr15:97989300:AC/A	- 1 bp	40976	2	0.0 %	20488	2	0.01 %	0.01 %
GUIDE3_OFFTARGET10	WT	0	43542	43516	99.94 %	21771	21745	99.88 %	0.0 %
GUIDE3_OFFTARGET10	chr16:8806799:CA/C	- 1 bp	43542	8	0.02 %	21771	8	0.04 %	0.04 %
GUIDE3_OFFTARGET10	chr16:8806802:AG/A	- 1 bp	43542	6	0.01 %	21771	6	0.03 %	0.03 %
GUIDE3_OFFTARGET10	chr16:8806818:GA/G	- 1 bp	43542	6	0.01 %	21771	6	0.03 %	0.03 %
GUIDE3_OFFTARGET10	chr16:8806829:GA/G	- 1 bp	43542	2	0.0 %	21771	2	0.01 %	0.01 %
GUIDE3_OFFTARGET10	chr16:8806798:TC/T	- 1 bp	43542	1	0.0 %	21771	1	0.0 %	0.0 %
GUIDE3_OFFTARGET10	chr16:8806795:CT/C	- 1 bp	43542	1	0.0 %	21771	1	0.0 %	0.0 %
GUIDE3_OFFTARGET10	chr16:8806802:A/AG	+ 1 bp	43542	1	0.0 %	21771	1	0.0 %	0.0 %
GUIDE3_OFFTARGET10	chr16:8806828:TG/T	- 1 bp	43542	1	0.0 %	21771	1	0.0 %	0.0 %
GUIDE3_OFFTARGET38	WT	0	42466	42379	99.8 %	21233	21146	99.59 %	0.0 %
GUIDE3_OFFTARGET38	chr16:50378318:GA/G	- 1 bp	42466	21	0.05 %	21233	21	0.1 %	0.1 %
GUIDE3_OFFTARGET38	chr16:50378341:TC/T	- 1 bp	42466	18	0.04 %	21233	18	0.08 %	0.08 %
GUIDE3_OFFTARGET38	chr16:50378344:AG/A	- 1 bp	42466	14	0.03 %	21233	14	0.07 %	0.07 %
GUIDE3_OFFTARGET38	chr16:50378351:CT/C	- 1 bp	42466	13	0.03 %	21233	13	0.06 %	0.06 %
GUIDE3_OFFTARGET38	chr16:50378314:CT/C	- 1 bp	42466	6	0.01 %	21233	6	0.03 %	0.03 %
GUIDE3_OFFTARGET38	chr16:50378322:AC/A	- 1 bp	42466	4	0.01 %	21233	4	0.02 %	0.02 %
GUIDE3_OFFTARGET38	chr16:50378343:CA/C	- 1 bp	42466	3	0.01 %	21233	3	0.01 %	0.01 %
GUIDE3_OFFTARGET38	chr16:50378339:CT/C	- 1 bp	42466	2	0.0 %	21233	2	0.01 %	0.01 %
GUIDE3_OFFTARGET38	chr16:50378348:GC/G	- 1 bp	42466	2	0.0 %	21233	2	0.01 %	0.01 %
GUIDE2_OFFTARGET8	WT	0	42366	42323	99.9 %	21183	21140	99.8 %	0.0 %
GUIDE2_OFFTARGET8	chr17:62931396:CA/C	- 1 bp	42366	12	0.03 %	21183	12	0.06 %	0.06 %
GUIDE2_OFFTARGET8	chr17:62931369:AG/A	- 1 bp	42366	8	0.02 %	21183	8	0.04 %	0.04 %
GUIDE2_OFFTARGET8	chr17:62931399:AG/A	- 1 bp	42366	6	0.01 %	21183	6	0.03 %	0.03 %
GUIDE2_OFFTARGET8	chr17:62931363:CA/C	- 1 bp	42366	4	0.01 %	21183	4	0.02 %	0.02 %
GUIDE2_OFFTARGET8	chr17:62931381:GA/G	- 1 bp	42366	3	0.01 %	21183	3	0.01 %	0.01 %
GUIDE2_OFFTARGET8	chr17:62931368:CA/C	- 1 bp	42366	1	0.0 %	21183	1	0.0 %	0.0 %
GUIDE2_OFFTARGET8	chr17:62931379:AT/A	- 1 bp	42366	1	0.0 %	21183	1	0.0 %	0.0 %
GUIDE2_OFFTARGET8	chr17:62931382:AG/A	- 1 bp	42366	1	0.0 %	21183	1	0.0 %	0.0 %
GUIDE2_OFFTARGET8	chr17:62931369:A/AT	+ 1 bp	42366	1	0.0 %	21183	1	0.0 %	0.0 %
GUIDE2_OFFTARGET5	WT	0	43270	43199	99.84 %	21635	21564	99.67 %	0.0 %
GUIDE2_OFFTARGET5	chr18:70473614:TC/T	- 1 bp	43270	11	0.03 %	21635	11	0.05 %	0.05 %
GUIDE2_OFFTARGET5	chr18:70473611:CTT/C	- 2 bp	43270	9	0.02 %	21635	9	0.04 %	0.04 %
GUIDE2_OFFTARGET5	chr18:70473618:CT/C	- 1 bp	43270	8	0.02 %	21635	8	0.04 %	0.04 %
GUIDE2_OFFTARGET5	chr18:70473631:GT/G	- 1 bp	43270	7	0.02 %	21635	7	0.03 %	0.03 %
GUIDE2_OFFTARGET5	chr18:70473604:CCT/C	- 2 bp	43270	6	0.01 %	21635	6	0.03 %	0.03 %
GUIDE2_OFFTARGET5	chr18:70473611:CT/C	- 1 bp	43270	6	0.01 %	21635	6	0.03 %	0.03 %
GUIDE2_OFFTARGET5	chr18:70473601:CA/C	- 1 bp	43270	4	0.01 %	21635	4	0.02 %	0.02 %
GUIDE2_OFFTARGET5	chr18:70473623:CT/C	- 1 bp	43270	4	0.01 %	21635	4	0.02 %	0.02 %
GUIDE2_OFFTARGET5	chr18:70473626:TC/T	- 1 bp	43270	3	0.01 %	21635	3	0.01 %	0.01 %
GUIDE2_OFFTARGET6	WT	0	43364	43344	99.95 %	21682	21662	99.91 %	0.0 %
GUIDE2_OFFTARGET6	chr21:30330283:GA/G	- 1 bp	43364	9	0.02 %	21682	9	0.04 %	0.04 %
GUIDE2_OFFTARGET6	chr21:30330306:AC/A	- 1 bp	43364	5	0.01 %	21682	5	0.02 %	0.02 %
GUIDE2_OFFTARGET6	chr21:30330303:G/GA	+ 1 bp	43364	1	0.0 %	21682	1	0.0 %	0.0 %
GUIDE2_OFFTARGET6	chr21:30330300:GA/G	- 1 bp	43364	1	0.0 %	21682	1	0.0 %	0.0 %
GUIDE2_OFFTARGET6	chr21:30330289:AG/A	- 1 bp	43364	1	0.0 %	21682	1	0.0 %	0.0 %
GUIDE2_OFFTARGET6	chr21:30330272:TA/T	- 1 bp	43364	1	0.0 %	21682	1	0.0 %	0.0 %
GUIDE2_OFFTARGET6	chr21:30330288:GA/G	- 1 bp	43364	1	0.0 %	21682	1	0.0 %	0.0 %
GUIDE2_OFFTARGET6	chr21:30330303:GA/G	- 1 bp	43364	1	0.0 %	21682	1	0.0 %	0.0 %
GUIDE3_OFFTARGET41	WT	0	41622	41560	99.85 %	20811	20749	99.7 %	0.0 %
GUIDE3_OFFTARGET41	chrX:10931944:TG/T	- 1 bp	41622	32	0.08 %	20811	32	0.15 %	0.15 %
GUIDE3_OFFTARGET41	chrX:10931910:CA/C	- 1 bp	41622	7	0.02 %	20811	7	0.03 %	0.03 %
GUIDE3_OFFTARGET41	chrX:10931934:AG/A	- 1 bp	41622	5	0.01 %	20811	5	0.02 %	0.02 %
GUIDE3_OFFTARGET41	chrX:10931918:GC/G	- 1 bp	41622	4	0.01 %	20811	4	0.02 %	0.02 %
GUIDE3_OFFTARGET41	chrX:10931928:GGA/G	- 2 bp	41622	3	0.01 %	20811	3	0.01 %	0.01 %
GUIDE3_OFFTARGET41	chrX:10931906:CT/C	- 1 bp	41622	2	0.0 %	20811	2	0.01 %	0.01 %
GUIDE3_OFFTARGET41	chrX:10931936:GA/G	- 1 bp	41622	2	0.0 %	20811	2	0.01 %	0.01 %
GUIDE3_OFFTARGET41	chrX:10931920:CA/C	- 1 bp	41622	2	0.0 %	20811	2	0.01 %	0.01 %
GUIDE3_OFFTARGET41	chrX:10931930:AG/A	- 1 bp	41622	1	0.0 %	20811	1	0.0 %	0.0 %
GUIDE3_OFFTARGET34	WT	0	42274	42206	99.84 %	21137	21069	99.68 %	0.0 %
GUIDE3_OFFTARGET34	chrX:56400966:TG/T	- 1 bp	42274	17	0.04 %	21137	17	0.08 %	0.08 %
GUIDE3_OFFTARGET34	chrX:56400931:AT/A	- 1 bp	42274	17	0.04 %	21137	17	0.08 %	0.08 %
GUIDE3_OFFTARGET34	chrX:56400952:TG/T	- 1 bp	42274	12	0.03 %	21137	12	0.06 %	0.06 %
GUIDE3_OFFTARGET34	chrX:56400963:TG/T	- 1 bp	42274	7	0.02 %	21137	7	0.03 %	0.03 %
GUIDE3_OFFTARGET34	chrX:56400955:GT/G	- 1 bp	42274	3	0.01 %	21137	3	0.01 %	0.01 %
GUIDE3_OFFTARGET34	chrX:56400965:GT/G	- 1 bp	42274	3	0.01 %	21137	3	0.01 %	0.01 %
GUIDE3_OFFTARGET34	chrX:56400935:TC/T	- 1 bp	42274	2	0.0 %	21137	2	0.01 %	0.01 %
GUIDE3_OFFTARGET34	chrX:56400954:GGT/G	- 2 bp	42274	2	0.0 %	21137	2	0.01 %	0.01 %
GUIDE3_OFFTARGET34	chrX:56400930:CA/C	- 1 bp	42274	1	0.0 %	21137	1	0.0 %	0.0 %
GUIDE2_OFFTARGET12	WT	0	40612	40513	99.76 %	20306	20207	99.51 %	0.0 %
GUIDE2_OFFTARGET12	chrX:112477503:TG/T	- 1 bp	40612	66	0.16 %	20306	66	0.33 %	0.33 %
GUIDE2_OFFTARGET12	chrX:112477482:CA/C	- 1 bp	40612	18	0.04 %	20306	18	0.09 %	0.09 %
GUIDE2_OFFTARGET12	chrX:112477502:GT/G	- 1 bp	40612	5	0.01 %	20306	5	0.02 %	0.02 %
GUIDE2_OFFTARGET12	chrX:112477508:GA/G	- 1 bp	40612	3	0.01 %	20306	3	0.01 %	0.01 %
GUIDE2_OFFTARGET12	chrX:112477503:T/TG	+ 1 bp	40612	2	0.0 %	20306	2	0.01 %	0.01 %
GUIDE2_OFFTARGET12	chrX:112477499:CA/C	- 1 bp	40612	2	0.0 %	20306	2	0.01 %	0.01 %
GUIDE2_OFFTARGET12	chrX:112477503:T/TA	+ 1 bp	40612	1	0.0 %	20306	1	0.0 %	0.0 %
GUIDE2_OFFTARGET12	chrX:112477496:AG/A	- 1 bp	40612	1	0.0 %	20306	1	0.0 %	0.0 %
GUIDE2_OFFTARGET12	chrX:112477503:TGG/T	- 2 bp	40612	1	0.0 %	20306	1	0.0 %	0.0 %

Advanced Tab

Top on-target variants

The 10 most frequent variants for each on-target. Each row represents either a wildtype (WT) allele or a variant allele within the indicated on-target. The alleles are sorted in descending order by frequency (# Alleles), with on-targets stacked on top of each other. The columns of this table are described below:

Target: the on-target name specified in the Target column of the target_groups.csv file.

Variant: indicates the genotype of the allele. It may be wildtype (WT) or edited. A variant is in the format of chromosome:position:reference bases/alternative bases.
Example:
chr2:241852772:G/GGGTACCCC indicates that at position 241852772 in chromosome 2, there was an 8bp insertion, changing G to GGGTACCCC.

Modification: The type of change present in the allele. In the Knockout Report, these are specified as 0 (identical to the reference sequence) or +/- [X] bp for indels.>

Total alleles: The total number of alleles that meet all the criteria in the "Default variant filters" section (above) for the target. This number is twice the value of "Total cells" (see definition below) since the pipeline assumes all cells as diploid.

# alleles: The number of alleles carrying the specific variant.

% alleles: The percentage of alleles carrying the specific variant, as calculated by: (# alleles/ total alleles) *100

Total cells: The total number of cells that meet all the criteria in the "Default variant filters" section (above) for the target.

# cells: number of cells carrying the specific variant.

% cells: The percentage of cells carrying the specific variant, as calculated by (# cells/ total cells) *100

% heterozygous: The percentage of cells with the variant on one allele (mono-allelic edit).

% homozygous: The percentage of cells with the variant on both alleles (bi-allelic edit).

Top off-target variants

The 10 most frequent variants for each predicted off-target. Each row represents either a wildtype (WT) allele or a variant within the indicated off-target. The alleles are sorted in descending order by frequency (# alleles), with off-targets stacked on top of each other. The columns of this table are the same as the descriptions for the "Top on-target variants" table.

On-target INDEL lengths

The percentage and number of alleles containing particular insertion or deletion (indel) lengths for an on-target edit. Indels of the same length are grouped together irrespective of their coordinates or sequences. For visualization purposes, the indel lengths were limited to 35 bp. The percentages were calculated as: (# indels of Xbp in length/ total edited alleles) *100). Variant length was calculated by subtracting the alternative bases from the reference bases. Negative values represent deletions and positive values represent insertions. If there are multiple on-targets in the same sample, the on-target names may be selected from the drop-down menu.

Zygosity of on-target edits

The number and percentage of cells with wildtype (WT), mono-allelic, or bi-allelic edits (assuming cells are diploid). If there are multiple on-targets in the same sample, the on-target names may be selected from the drop-down menu.

On-target indel start position

The distribution of start positions of insertion or deletions (indels) within the on-target activity window. The chromosome position (x-axis) represents the first nucleotide position of the indels. Only positions with >=1 variant found are shown. Each bar represents a frequency value at that position. If there are multiple on-targets in the same sample, the on-target names may be selected from the drop-down menu.

Zygosity of top 10 on-target variants

The 10 most frequent variants and the percentage of cells that contain the variant on both alleles (homozygous), one allele (heterozygous), and not at all (wildtype, WT). The variant name (x-axis) is in the format of "chromosome:position:reference bases/alternative bases." If there are multiple on-targets in the same sample, the on-target names may be selected from the drop-down menu.

Top edit combinations per group

The most frequent combinations of co-occurring on-target(s) and/or off-target(s) within each Group. The percentage values were calculated by: (the number of cells in each combination / total number of cells that meet the criteria in the "Default variant filters" section for on-target(s) and/or off-target(s)) *100. The table will display a maximum of 5 combinations per Group.

Top 5 edit sites per group

The percentage of cells containing the top edit sites for each Group (up to 5). A Group encompasses the on-target edit and any associated predicted off-target edits. The Group names are designated in the "Group" column of the target group file (target_groups.csv).

Co-occurrence of on-target edits

Note: This plot is only relevant if multiple on-targets are specified in the target_groups.csv file.
The percentage of cells with the most frequent combinations of multiple on-target edits. The plot contains two parts. The upper portion is a bar plot with each bar representing the percentage of cells with each combination of edits. The lower portion is a heatmap. Each row of the heatmap represents an on-target. Each color square represents zygosity: WT (red), mono-allelic edit (purple), bi-allelic edit (green).

Protein (optional)

The number of cells with different combinations of edits and cell-surface protein expression. Each column represents cells with the indicated combination of edits and protein expression. Top: the number of cells with each combination. Middle: the zygosity of on-target and predicted off-target edits. Each color square represents zygosity: WT (red), mono-allelic edit (purple), bi-allelic edit (green). Bottom: expression of cell-surface proteins.